电子邮箱：peiyan3@swu.edu.cn
电话：023-68251883
通讯地址：重庆市北碚区天生路2号西南大学生物技术中心（南校区隆平楼506室）
研究兴趣
l 棉花基因工程育种
l 杀虫真菌分子改良
Research Emphasis
One goal of research in my lab is to understand the genetic and molecular basis of cotton fiber development. Cotton is truly a miracle crop in the world, providing fibers for textile manufacturing and affording oil and protein for human food and animal feed production. Cotton fibers are single cells resulted from elongated cells of the ovule epidermis and undergo rapid and synchronous elongation and are considered as an ideal system for studying cell elongation and cell wall development. Fiber number and fiber length, fiber fineness and fiber strength are important agronomy traits for cotton breeding, which are greatly determined by fiber cell initiation, elongation and secondary cell wall accumulation. However, so far, knowledge of the regulatory mechanism controlling cotton fiber initiation and elongation is still mostly unknown. Currently, we are trying to elucidate how plant hormones and their interactions regulate cotton fiber cell development and how to manipulate the expression of the plant hormone biosynthesis and signaling genes to improve cotton fiber quality and yield. We found that auxin, a key phytohormone involved in controlling many aspects of plant development, accumulates highly and specifically in the initiating fiber cells. Based on this observation, we hypothesized that enhancing IAA level in the ovule epidermis at the time of fiber cell initiation by the tissue-specific expression of an IAA biosynthetic gene would promote the initiation of fiber cells and thereby increase fiber yield. Through this way, we generated transgenic cottons which produced higher yield and better quality fibers. The results had been published in “Nature Biotechnology”, a high level journal, and a comment in the same volume gave high words to our work “this study illustrates the promise of the next generation of transgenic crops.”
As transgenic crops are rapidly becoming a staple of modern agriculture in the world, the food safety and gene flow problems resulted from genetically modified crops have been a great concern to both the public and the scientific community. To address this problem, we developed a new recombination system characterized with highly efficient in deleting all functional transgenes from the pollen, seed or both of tobacco plants. This system can be applicable for vegetatively propagated plants. We are now engaged in establishing a new two-component transcript activation system for gene deletion in hybrid crops, such as rice, maize and canola.
Another group in my laboratory is interested in molecular basis of insect fungal diseases. Biological control agents are being considered as supplements or alternatives to synthetic chemical insecticides that are known to have toxic effects on nontarget organisms, including animals and humans. Entomopathogenic fungi are key regulatory factors of insect populations in nature and considered as the only practical microbial control for insects that feed by sucking plant and animal juice and for the many acridid and coleopteran pests which have no known viral or bacterial pathogens. We had cloned several cuticle-degrading enzyme genes, e.g., proteinase or chitinase genes, from Beauveria bassiana, a widely used entomopathogenic fungi in insect pest control. Then, we utilized a chitin-binding domain from an insect, the silkworm moth (B. mori), to produce an engineered chitinase and proteinase in the insect pathogen B. bassiana. The engineered chitinase and proteinase showed increased kinetic properties in substrate hydrolysis than those of the wild-type chitinase and proteinase. Furthermore, when the engineered chitinase and proteinase were overexpressed in B. bassiana, respectively, the genetically modified fungus showed an increase in virulence over a strain with a constitutively expressed native chitinase or proteinase gene. We have demonstrated a strategy where genetic components of the host insect can be incorporated into the fungal pathogen in order to increase host cuticle penetration ability. To isolate new genes involved in infection, we have set up a T-DNA insertion mutant pool of B. bassiana. Some interesting genes had been cloned and characterized from this pool.
承担科研项目
1. 国家转基因生物新品种培育重大专项，2016ZX08005--003-004， 棉花优质纤维基因的挖掘及分子改良，2016.1.1-2020.12.31，347万元。
2. 国家科技部重点研发计划，2016YFD100505，棉花纤维品质相关基因和调控元件的克隆与鉴定，2016.7-2020.12，120万元。
3. 国家转基因生物新品种培育重大专项，棉花优质纤维基因的挖掘及材料创新，2014.1-2015.12，148万元。
4. 国家转基因生物新品种培育重大专项，2013ZX08005003-005，转基因优质纤维棉花新品种培育，2013年，105万元。
5. 国家自然科学基金重点项目，31130039，棉花纤维-种子协同改良的分子基础，2012/01-2016/12，320万元。
6. 国家自然科学基金重点项目，3053049，利用激素合成与传导基因改良棉花纤维质与产量，2006/01-2009/12，180万元。
7. 国家重点基础研究发展规划项目（973）项目，2010CB126000，棉花纤维品质功能基因组学研究与分子改良，2010 -2014，220万元。
8. 国家重点基础研究发展规划项目（973）项目，2003CB114203，球孢白僵菌侵染与抗逆相关功能基因的研究，2003-2008年， 280万元。
9. 国家重点基础研究发展规划项目（973）项目，2009CB118904，害虫生防真菌致病与抗逆的分子机理、遗传改良及应用，2009-2010年，150万元。
10. 国家高技术研究发展计划（863）计划，2006AA10A212，“农业生物药物”重大项目：细菌、真菌类生物杀虫剂研究与创制，2007-2011年，63万元。
11. 国家转基因生物新品种培育重大专项，2009ZX08005-023B，高衣分转基因棉花的创建与新品种选育，2009-2011年，500万元。
12. 国家转基因生物新品种培育重大专项，2008ZX08003-003，转基因优质纤维棉花新品种培育，2008-2010年，240万元。
13. 重庆市科，2002-7592，高效无公害微生物农药的研制与中试，2002-2005，100万。
14. 国家科技部科技攻关项目，ZJY-A-01，广谱抗真菌双价基因的筛选及棉花抗黄萎病的基因工程，1999.9-2002.12，100万元。
发表论文（部分）
1. Li X; Ouyang X; Zhang Z; He L; Wang Y; Li Y; Zhao J; Chen Z; Wang C; Ding L; Pei Y; Xiao Y*, Over-expression of the red plant gene R1 enhances anthocyanin production and resistance to bollworm and spider mite in cotton. Molecular Genetics and Genomics, 2019, doi:10.1007/s00438-018-1525-3
2. Zhang M, Zeng J, Cao H, Pei Y, YC3.60-Based Imaging Analysis on Calcium Level in Cotton Cells. In B Zhang, ed, Transgenic Cotton: Methods and Protocols. Springer New York, New York, NY, 2019, pp 177-185
3. Yan Qian#; Wang Yi#; Li Qian; Zhang Zhengsheng; Ding Hui; Zhang Yue; Liu Housheng; Luo Ming; Liu Dexin; Song Wu; Liu Haifeng; Yao Dan; Ouyang Xufen;Li Yaohua; Li Xin; Pei Yan; Xiao Yuehua*, Upregulation of GhTT2-3A in cotton fibers during secondary wall thickening results in brown fibers with improved quality, Plant Biotechnology Journal 2018.10, 16:1735-1747.
4. Fan Yanhua, Liu Xi, Nemat O.Keyhani, Tang Guirong, Pei Yan, Zhang Wenwen, Tong Sheng, Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death, PNAS, 2017, 114 (9) E1578.
5. Zhang M, Cao H, Hou L, Song S, Zeng J, Pei Y. In vivo imaging of Ca2+ accumulation during cotton fiber initiation using fluorescent indicator YC3.60. Plant Cell Reports, 2017, 36: 911-918
6. Zhang M, Xiao Y, Zeng J, Pei Y. PIN-formed protein, a door to reveal the mechanism for auxin-triggered initiation of cotton fiber. Plant Signaling & Behavior, 2017, 12: e1319031
7. Zhang M, Zeng J, Long H, Xiao Y, Yan X, Pei Y. Auxin regulates cotton fiber initiation via GhPIN-mediated auxin transport. Plant and Cell Physiology, 2017, 58: 385-397
8. Huang S., He Z., Zhang S., Keyhani N.O., Song Y., Yang Z., Jiang Y., Zhang W., Pei Y., Zhang Y.* Interplay between calcineurin and the Slt2 MAP-kinase in mediating cell wall integrity, conidiation and virulence in the insect fungal pathogen Beauveria bassiana. Fungal Genet. Biol. 2015, 83: 78-91.
9. Yan Qian; Liu Housheng; Yao Dan; Li Xin; Chen Han; Dou Yang; Wang Yi; Pei Yan; Xiao Yuehua*, The basic/Helix-Loop-Helix protein family in Gossypium: Reference genes and their evolution during tetraploidization, Plos One, 2015.5.18, 10(5): e0126558
10. Zhao Juan, Bai Wenqin, Zeng Qiwei, Song Shuiqing, Zhang Mi, Li Xianbi, Hou Lei, Xiao Yuehua, Luo Ming, Li Demou, Luo Xiaoying, Pei Yan*, Moderately enhancing cytokinin level by down-regulation of GhCKX expression in cotton concurrently increases fiber and seed yield, Mol Breeding, 2015, 35(2):60
11. Bai Wenqin#; Xiao Yuehua#; Zhao Juan; Song Shuiqing; Hu Lin; Zeng Jianyan; Li Xianbi; Hou Lei; Luo Ming; Li Demou; Pei Yan*, Gibberellin overproduction promotes sucrose synthase expression and secondary cell wall deposition in cotton fibers, PLos One, 2014.5.9, 9(5): e96537
12. Yang Linzhi, Nemat O. Keyhani, Tang Guirong, Tian Chuang, Lu Ruipeng, Wang Xin, Pei Yan, Fan Yanhua. Expression of a Toll Signaling Regulator Serpin in a Mycoinsecticide for Increased Virulence. Appl Environ Microbiol, 2014, 80 (15): 4531-4539
13. Zhou YH, Hou L, Zhang YJ, Fan YH, Luo ZB, Jin D, Zhou QS, Li YJ, Wang Y, Pei Y. Expression and promoter characterization of BbPacC, a pH response transcription factor gene of the entomopathogenic fungus Beauveria bassiana. Microbiology. 2014 Feb;160(Pt 2):353-61.
14. Xiao Yuehua#*; Yan Qian; Ding Hui; Luo Ming; Hou Lei; Zhang Mi; Yao Dan; Liu Housheng; Li Xin; Zhao Jia; Pei Yan, Transcriptome and biochemical analyses revealed a detailed proanthocyanidin biosynthesis pathway in brown cotton fiber , PLos One, 2014.1.21, 9(1): e86344
15. Zhou Yonghong, Zhang Yongjun, Luo Zhibing, Fan Yanhua, Tang Guirong, Liu Liji, Pei Yan*, Selection of optimal reference genes for expression analysis in the entomopathogenic fungus Beauveria bassiana during development, under changing nutrient conditions, and after exposure to abiotic stresses, Appl Microbiol Biotechnol, 2012 Jan;93(2):679-85.
16. Xiao Yuehua#; Pei Yan*, Asymmetric overlap extension PCR method for site-directed mutagenesis, Methods Mol Biol., 2011;687:277-82
17. Zhang Mi; Zheng Xuelian; Song Shuiqing; Zeng Qiwei; Hou Lei; Li Demou; Zhao Juan; Wei Yuan; Li Xianbi; Luo Ming; Xiao Yuehua; Luo Xiaoying; Zhang Jinfa; Xiang Chengbin; Pei Yan*, Spatiotemporal manipulation of auxin biosynthesis in cotton ovule epidermal cells enhances fiber yield and quality, Nature Biotechnology, 2011.5, 29(5): 453~458
18. Gao Q, Jin K, Ying SH, Zhang Y, Xiao G, Shang Y, Duan Z, Hu X, Xie XQ, Zhou G, Peng G, Luo Z, Huang W, Wang B, Fang W, Wang S, Zhong Y, Ma LJ, St Leger RJ, Zhao GP, Pei Y, Feng MG, Xia Y, Wang C, Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum. PLoS Genetics, 2011, 7: 1-18
19. Jin Kai, Luo Zhibing, Jiang Xiaodong, Zhang Yongjun, Zhou Yonghong, Pei Yan * Carbon catabolite repressor gene BbCre1 i, , , nfluences carbon source uptake but does not have a big impact on virulence in Beauveria bassiana. Journal of Invertebrate Pathology 106 (2011) 400–406
20. Xiao Yuehua; Li Demou; Yin Menghui; Li Xianbi; Zhang Mi; Wang Yanjun; Dong Jing; Zhao Juan; Luo Ming; Luo Xiaoying; Hou Lei; Hu Lin; Pei Yan*, Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis , Journal of Plant Physiology, 2010, 167(10): 829~837
21. Zhang Yongjun, Zhang Jianqing, Jiang Xiaodong, Wang Guijiang, Luo Zhibing, Fan Yanhua, Wu Zengqiang, and Pei Yan*,Requirement of , a Mitogen-Activated Protein Kinase for Appressorium Formation and Penetration of Insect Cuticle by the Entomopathogenic Fungus Beauveria bassiana Applied and Environmental Microbiology, 2010, 76(7):2262-2270
22. Jin Kai, Zhang Yongjun, Fang Weiguo, Luo Zhibing, Zhou Yonghong, Pei Yan*, Carboxylate Transporter Gene JEN1 from the Entomopathogenic Fungus Beauveria bassiana Is Involved in Conidiation and Virulence. Applied and Environmental Microbiology, 2010, 76(1):254-263
23. Fan Yanhua, Pei Xiaoqiong, Guo Shujuan, Zhang Yongjun, Luo Zhibing, Liao Xinggang, Pei Yan*, Increased virulence using engineered protease-chitin binding domain hybrid expressed in the entomopathogenic fungus Beauveria bassiana Microbial Pathogenesis, 2010, 49: 376-380.
24. Zhang Yongjun, Zhao Jianhua, Fang Weiguo, Zhang Jianqing, Luo Zhibing, Zhang Mi, Fan Yanhua, Pei Yan*, Mitogen-Activated Protein Kinase hog1 in the Entomopathogenic Fungus Beauveria bassiana Regulates Environmental Stress Responses and Virulence to Insects, Applied and Environmental Microbiology, 2009, 75:3787-3795
25. Ma Jincheng, Zhou Qun, Zhou Yonghong, Liao Xinggang, Zhang Yongjun, Jin Dan, Pei Yan *,The size and ratio of homologous sequence to non-homologous sequence in gene disruption cassette influences the gene targeting efficiency in Beauveria bassiana, Appl Microbiol Biotechnol, 2009, 82:891–898
26. Zhang Yongjun, Li Zunhua, Luo Zhibing, Zhang Jianqing, Fan Yanhua, Pei Yan*, Light stimulates conidiation of the entomopathogenic fungus Beauveria bassiana, Biocontrol Science and Technology, 2009,19 (1): 91-101
27. Dong J, Yin MH, Yang F, Zhao J, Qin S, Hou L, Luo M, Pei Y, Xiao YH*, Cloning and expression profile of gibberellin insensitive dwarf GID1 homologous genes from cotton. Acta Agron Sin, 2009, 35(10): 1822-1830.
28. Zou Xiuping, Li Demou, Luo Xiaoying, Luo Keming, Pei Yan*. An improved procedure for Agrobacterium-mediated transformation of trifoliate orange (Poncirus trifoliate L. Raf.) via indirect organogenesis, In Vitro Cell.Dev. Biol.-Plant, 2008, 44:169–177
29. Hou L, Liu H, Li J, Yang X, Xiao Y, Luo M, Song S, Yang G, Pei Y*, SCFP, a novel fiber-specific promoter in cotton. Chin. Sci. Bull. 2008, 53, 2639-264
30. Luo Keming, Deng Wei, Xu Shan, Pei Yan*, Functional analysis of the Arabidopsis thaliana poly(A) binding protein PAB5 gene promoter in Nicotiana tabacum. Plant Cell Rep, 2008, 27:1811-1819
31. Jin Kai, Zhang Yongjun, Luo Zhibing, Xiao Yuehua, Fan Yanhua, Wu Di, Pei Yan*, An improved method for Beauveria bassiana transformation using phosphinothricin acetlytransferase and green fluorescent protein fusion gene as a selectable and visible marker Biotechnol Lett, 2008, 30:1379–1383
32. Luo M, Xiao Y, Li X, Lu X, Deng W, Li D, Hou L, Hu M, Li Y, Pei Y. GhDET2, a steroid 5α-reductase, plays an important role in cotton fiber cell initiation and elongation. Plant J 2007, 51, 419-430
33. Xiao YH, Yin MH, Hou L, Luo M, Pei Y*. Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis. Biotechnol. Let. 2007, 29: 925-930
34. Xiao YH, Peng Y, Luo M, Li DM, Hou L, Pei Y*. Sequential amplification of flanking sequences by Y-shaped adaptor dependent extension using multiple templates. J. Plant Physiol. Mol. Bio. 2007, 33(1): 85-90
35. Xiao YH, Li XB, Yang XY, Luo M, Hou L, Guo SH, Luo XY, Pei Y*. Cloning and characterization of a balsam pear class I chitinase gene (Mcchit1) and its ectopic expression enhances fungal resistance in transgenic plants. Biosci Biotechnol Biochem. 2007, 71, 1211-1219
36. Xiao Yuehua; Zhang Zhengsheng; Yin Menghui; Luo Ming; Li Xianbi; Hou Lei; Pei Yan*, Cotton flavonoid structural genes related to the pigmentation in brown fibers , Biochemical and Biophysical Research Communications, 2007.6.22, 358(1): 73~78
37. Xiao YH, Zhang ZS, Yin MH, Luo M, Li X, Hou L, Pei Y*. Cotton flavonoid structural genes related to the pigmentation in brown fibers. Biochem. Biophys. Res. Commun. 2007, 358(1): 73-78
38. Yang X, Xiao Y, Wang X, Li J，Pei Y. Expression of a novel small antimicrobial protein from the seeds of motherwort (Leonurus japonicus) confers disease resistance in tobacco. Appl Environ Microbiol 2007, 73: 939-946
39. Xiao YH, Yin MH, Hou L, Pei Y*. Direct amplification of intron-containing hairpin RNA construct from genomic DNA. Bio Techniques 2006, 41: 548-552
40. Xiao YH, Ye YF, Feng Y, Li XB, Luo M, Hou L, Luo XY, Li DM, Pei Y*. Functional expression of the cotton gibberellin acid oxidase homologous gene GhGA20ox1 in tobacco. J. Plant Physiol. Mol. Bio. 2006, 32: 563-569
41. Luo K, Deng W, Xiao Y, Zheng X, Li Y, Pei Y. Leaf senescence is delayed in tobacco plants expressing the maize knotted1 gene under the control of a wound-inducible promoter. Plant Cell Rep. 2006, 25(11):1246-1254
42. Luo K, Zheng X, Chen Y, Xiao Y, Zhao D, McAvoy R, Pei Y, Li Y. The maize Knotted1 gene is an effective positive selectable marker gene for Agrobacterium- mediated tobacco transformation. Plant Cell Reports 2006, 25: 403-409
43. Zhang Z, Xiao Y, Luo M, Li X, Luo X, Hou L, Li D, Pei Y. Construction of genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium lirsutum L.). Euphytica, 2005, 144: 91-99
44. Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol. 2005, 71(1): 363-370
45. He G, Hou L, Xiao Y, Luo X, Niu G, Yang G, Pei Y. A common sequence difference between cytoplasmic male sterile lines and their maintainer lines existing in rice (Oryza sativa L.) chloroplast tRNA-Leu gene region. Euphytica, 2003, 131(3): 269-274
*: Corresponding author
科研成果
专利申请
1. 裴炎，范艳华，张永军，方卫国. 重组丝氨酸蛋白酶及含有其的杀虫真菌剂（专利号：ZL200510114696.1）
2. 裴炎，周国庆，侯磊，罗小英，杨光伟，杨星勇，李德谋，肖月华，李名扬. 几丁质酶和抗菌肽基因双价抗病载体pChima（专利号：ZL99119241.9）
3. 裴炎，侯磊，李德谋，宋水清，李先碧，罗明，肖月华，郑雪莲，曾其伟，张觅，邱坤，罗凤涛，表达生长素合成相关基因的植物表达载体及其在棉花纤维性状改良的应用（专利申请号：200810142518.3）
4. 张永军，裴炎，杨星勇，方卫国. 一种杀虫真菌分生孢子的液体保存方法（专利号： ZL01124159.4）
5. 张永军，罗志兵，裴炎. 一种真菌孢子可湿性粉剂（专利号：ZL200510080659.3）
6. 张永军，裴炎，彭荣. 通过降低培养基的水活度生产杀虫真菌液体种子和分生孢子的方法（专利：ZL200510085036.5）